What Is The Role Of Dna Ligase During Replication – An enzyme is a catalyst or chemical produced by cells to speed up a biochemical reaction. It is usually a protein molecule with a characteristic amino acid sequence that folds to form a three-dimensional structure that gives the molecule its unique properties. Another molecule with catalytic activity is the ribozyme, an enzyme made of RNA rather than protein. Enzymes can be classified and named according to the reaction they catalyze: (1) oxidoreductases, (2) transferases, (3) lyases, (4) isomerases, and (6) ligases. Basically, ligases are a group of enzymes that catalyze the binding of two molecules.

. In biology, ligase is a class of enzymes. But what does ligase do? Let’s define a ligase.

What Is The Role Of Dna Ligase During Replication

Ligase is a class of enzymes that cause two molecules to join or join. By definition in biology, it is a class of enzymes that catalyze the binding or coupling of two macromolecules to form new bonds such as C-O, C-N, and C-S. A ligase enzyme catalyzes a ligase reaction, e.g.

Nucleotide Excision Repair

Between complementary ends of DNA fragments. Thus, DNA ligase plays a critical role in DNA repair, replication, and recombination. Let us understand the mechanism of ligase using DNA Ligase as an example.

Biology/Biochemistry Definition: A ligase is an enzyme that catalyzes the joining of two molecules. An example is DNA ligase, which joins two DNA fragments to form a phosphodiester bond. Ligases are classified into six subclasses: (1) EC 6.1 (ligases that form carbon-oxygen bonds), (2) EC 6.2 (ligases that form carbon-sulfur bonds), (3) EC 6.3 (ligases that form carbon-nitrogen bonds), (4) EC 6.4 (ligases that form carbon-carbon bonds), (5) EC 6.5 (ligases that form phosphoric acid ester bonds), and (6) EC 6.6 (ligases that form nitrogen-metal bonds).

DNA ligase is required for DNA replication as well as the DNA repair process. DNA ligase is widely used in laboratories for recombinant DNA experiments. The most widely used ligase in the science laboratory is DNA ligase.

DNA ligase links the 3′ hydroxyl ends of one nucleotide (or acceptor) to the 5′ phosphate end of another (donor) using two covalent phosphodiester bonds, performing the following steps:

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Generally, these ligases are named according to the substrate or macromolecules involved in the reaction; for example, amino acid-RNA ligase catalyzes the formation of a C-O bond between an amino acid and a transfer RNA.

“ligase that catalyzes the joining of two macromolecules using energy molecules obtained from the hydrolysis of nucleoside triphosphates (such as ATP, GTP, CTP, TTP, and UTP), such as the hydrolysis of adenosine triphosphate (ATP) molecules to adenosine diphosphate (ADP)”

There are six classes of enzymes, one of which is ligases. Other classes of enzymes include oxidoreductases, transferases, hydrolases, isomerases, and lyases. Ligase and lyase are closely related classes of enzymes. However, ligase and lyase are different classes of enzymes.

Based on the molecular bond catalyzed by the ligase enzyme, ligase can be divided into six subclasses (see Table 2).

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