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Description: Name Class Date Guided Inquiry • Skills Lab Chapter 13 lab From DNA to Protein Synthesis Problem What steps are involved in making a protein?
Protein Synthesis Transcription And Translation Lab Answer Key
Class Name Date Guided Question • Skills Lab Chapter 13 lab From DNA to Protein Synthesis Problem What steps are involved in making a protein? Introduction Before a protein can be made, the biochemical blueprint for its construction must be packaged and extracted from the DNA “library.” First, a specific DNA sequence that codes for a protein is transcribed onto the corresponding strand of mRNA. In eukaryotic cells, the mRNA then leaves the nucleus and enters the cytoplasm. Ill cell, the mRNA molecule attaches to the ribosome, where the anticodon of tRNA translates the mRNA into amino acids. The completed amino acid chain, or polypeptide, is then folded into its final shape as a protein. In this lab, you will model DNA transcription and mRNA translation while deciphering cryptic messages. Focus Skills Use Models, Pre-Lab Questions Sequence 1. Sequence Briefly describe the process you will use to decipher the messages. 2. Compare and contrast What role do codons play in protein synthesis? What are they used for in coded messages?3. Guess which six letters will not appear in coded messages? Give a reason for your answer. 77 Class Name Date Program Part A: How to Interpret Messages 1. Write the corresponding mRNA strand of the DNA sequence given below by finding the mRNA codon that corresponds to each DNA triplet, base by base. The mRNA strand is initiated from you. Complete the printout. DNA: TAC CGT TTT CTT ATT TAC ATA ACT CTG CGA ATG mRNA: AUG GCA AAA GAA UAA 2. Use Figure 1 to match each mRNA codon from Step 1 with its corresponding amino acid. When the codon is a “stop” codon, insert “stop” in the sequence. The amino acid sequence is started for you. Complete the translation. methionine, alanine, lysine, glutamic acid, stop, Figure 1 Mapping of mRNA codons to amino acids 78 Class Name Date3. Use the table to find the one-letter symbol for each amino acid in sequence from Step 2. The symbols will spell a common sentence or expression. The first word is for you. MakeAmino Acid-One Letter Amino Acid Symbol Alanine L Amino Acid Symbol K A Leucine M FArginine R Lysine P SAsparagine N Methionine TAspartic Acid D Phenylalanine WCysteine C Proline YGlutamic Acid E Serine VGlutamine Q Threonine Threonine Threonine Threonine Threonine Threonine Threonine Threonine Threonine Threonine Glutamine Threonine Threonine Threonine Threonine Threonine CysteineC ding Messages 4. Use program from Part A to explain the following messages. Remember to copy DNA messages from mRNA codons and convert the codons into amino acids. a. TGA CGA TTT CTC LAW ACA CGC GCG CTTb. GTA CTT ATT TAA AGC ATC CGT ATT AGT GGC ATAc. TAC CTC CTT TGA ATT TAC CTT ACT CGT TGT ATT AAA TAT CAG CTCd. TGT GTA CTT ACT GGG GAT CGC TTG ATT GTA CGG AGC ATC ACG GTG CGA TTG CCC CTT CTGe. TGT GTG CTC ACT AGA GTA TAG GGA ATT AGG CGG TAT GAC AGC ATC CGA TGC ACT CTG CGC ACC TTA 79 Class Name Date Analyze and Conclude 1. Apply Concepts How did you know which bases to use when writing the DNA sequence into the mRNA codon? 2. Guess the DNA sequence of the first message in Part B started with TGT CGA instead of TGA CGA. Would the message change? Why or why not? 3. Use Analogies Suppose some codons are mapped to two different amino acids? What would be the effect on your translation of coded messages? What would be the result of protein production? 4. Sequence During the production of proteins in the cell, what happens to the RNA strand before it leaves the nucleus? 5. Test and Revise What step would you add after you copy the DNA to make a complete model of protein synthesis? Expand Your Inquiry Create your own secret message using DNA triplets. Exchange messages with your classmates and try to solve the messages you receive. Remember that there is no triplet of letters B, J, O, U, X, and Z. You will need to be creative to come up with messages that use those letters. 80I just finished the Transformation/Translation Lab from Kim Foglia for my AP Bio class. Last year when we did it, we decided there was a lot of cutting and clicking involved and not enough time to be able to keep our eyes on the ideas. This year, I only gave the lab groups half a strand of DNA and then we put our DNA strands together. This was a huge time saver. It also helped that I laminated and cut all the RNA nucleotides, so the students didn’t have to do it (and ideally, I won’t do it again). We completed the translation process together. Then the students return to their groups and work on each part of the changes. But I still felt like it took too long. Next year, I will assign a different variable to each group and then have them report/present the results to the whole class.
Biology By The Math Mom: Transcription And Translation Modeling In Biology
For extra practice in the process of copying and translating, we’ll add a little competition and have copying/translating races. “Proteins” that are built will be words, with words substituted for amino acids. The idea came from working in the Foglia lab and seeing a pin on Pinterest of the AwesomeScience product. I looked up 5 word quotes and found several interesting ones at http://www.lifestalker.com/21-simple-yet-powerful-five-word-quotes/ and http://hubpages.com/education/5 -word-quotes . At first I was going to make my own tRNA molecules so that everything could be typed. As much as I love Google graphics, a few minutes in, I decided to use Foglia’s empty tRNA molecules and write the names and anti-codons for myself. I blamed them in the hope that I wouldn’t do it again.
The tRNA molecules are placed on a table at the front of the room, and as the students finish copying and are in the process of translation, they come and pick one tRNA molecule at a time that corresponds to the strand of mRNA they made. Students actually have a full page to write their transcriptions and translations and answer a few questions to highlight some of the important, but often overlooked bits of transcription and translation.
Here is a link to the Kim Foglia Transcription and Translation Lab. And here’s a link to the Download Version Races. In this document, the answer key is the first and each of the following pages is for each of the 7 different expressions. I think I caught all my typos in the DNA threads, but if I missed one, let me know. When we decided what we would do in class to help students understand the process of printing and translating several blog posts gave me inspiration. I had just done a translational translation lab from Kim Foglia at AP Bio and read these posts about making proteins with beads on the Science Matters blog. The motivation was to combine these two labs into one for my class.
I started the process backwards, by deciding which amino acids would be in the finished proteins. Since the pack of beads I already had at home had only 7 colors, there were only 7 types of amino acids in our proteins. The two designed proteins were identical. I wanted the students to be able to see that two strands of mRNA with different orders of nucleotides can produce the same protein because there are many codons that code for the same amino acid. Visuals are helpful during discussions of silent change.
Solution: Biology Chapter 14 Rna And Protein Synthesis Review Answer Key
Then, I wrote the mRNA code for each protein, making sure to use different codons for the amino acids in the corresponding proteins. Then I wrote the DNA code that would be written into our mRNA strands. I felt like reverse transcriptase like this point! Now that I had all the code I needed, it was time to prepare the model tools,
I made the proteins with beads and wrote them with a number and put them aside as an answer key for the students to look at when they finished. I used the DNA molecules sheet from the Biology Corner, and I used the RNA nucleotides from Kim Foglia’s lab. I colored each DNA nucleotide that we will use in the lab, laminated it, and put it in the correct order for each of the 4 strands of DNA that we will start with in the lab. I joined the DNA strip with two long strips of packing tape – one in the front and one in the back. I wanted to make sure it was very durable because I wanted it to last for several years (OK, to be honest, I want them to last
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